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. 2002 Apr;184(8):2181–2191. doi: 10.1128/JB.184.8.2181-2191.2002

FIG. 3.

FIG. 3.

Effect of the srtA mutation on surface anchoring of LPXTG proteins by GAS. (A) Dot blot analysis for surface localization of M6, protein F, ScpA, GRAB, and T6 proteins in JRS4 and srtA derivatives. Each strain was grown overnight in THY broth, and cell density was standardized to an optical density at 600 nm of 2.0. Individual spots represent 10-μl aliquots of twofold serial dilutions of each strain. (B) Western immunoblot analysis of JRS4 and srtA derivatives with monoclonal antibody 10B6. CW, cell wall fraction; S, culture supernatant fraction. Proteins were separated by SDS-PAGE on 4 to 12% gradient gels, transferred to nitrocellulose, and detected with 10B6 as described in Materials and Methods. The sizes of molecular mass standards are indicated to the left (in kilodaltons). The arrow to the right represents the position of mature M6 protein.