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Southern hybridization analysis for the presence of srtA (A) and srtB (B) genes in strains of GAS and other gram-positive bacteria. DNA was digested with HindIII and separated by 0.8% agarose gel electrophoresis. Following transfer to a nylon membrane, srtA- and srtB-homologous sequences were detected by hybridization with an internal fragment labeled with [α-32P]dCTP. Gp, group; NT, nontypeable.
