Alignment of the C-terminal amino acid sequences of GAS proteins containing an LPXTG motif. Alignments were performed using ClustalW. Spy designations are annotations from the genome sequence of the M1 GAS strain SF370 (16). The sequence of the corresponding region of the M6 protein analyzed in this study is identical to the M1 sequence (23). References for the sequences are as follows: SPy130, -416, -747, -843, -872, -1494, and -1972 (57); Scl2 (35, 52, 69); GRAB (54); Scl1 (34, 53); Fba (64); ScpA (7); M1 (20). Protein F (61) and T6 (57) sequences are from the M6 strain JRS4 and are not present in the M1 genome.