FIG. 1.

Adsorption of phage LL-H to L. delbrueckii subsp. lactis. (A) Adsorption of phage LL-H to ATCC 15808 (•) and its phage-resistant mutants ATCC 15808(Bes-1) (▴) and ATCC 15808(Ads-5) (▪). Late-log-phase bacteria were centrifuged and resuspended in buffer A at a concentration of 5 × 10⁸ cells per ml. Cells at 37°C were infected with phage LL-H at an MOI of about 0.1. (B) Adsorption of phage LL-H(Bes-1) (○) and the mutant LL-H-a21 (□) to ATCC 15808 cells overloaded with phage LL-H. For controls, adsorption buffer was added instead of phage LL-H, and adsorption of phage LL-H(Bes-1) (•) and LL-H-a21 (▪) was measured. For details, see Materials and Methods.