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. 2002 May;184(9):2370–2378. doi: 10.1128/JB.184.9.2370-2378.2002

FIG. 4.

FIG. 4.

Localization of GldA in wild-type cells and in gldF and gldG mutants. Cell fractions were examined for GldA by Western blot analysis. Odd-numbered lanes contain soluble fractions, while even-numbered lanes contain membrane fractions. An equal amount, based on total cell protein measurements prior to fractionation, was loaded in each lane. (A) Lanes 1 and 2, wild-type cells; lanes 3 and 4, CJ787 (Tn4351 insertion in gldF); lanes 5 and 6, CJ787 with pMK314, which carries gldFG; lanes 7 and 8, CJ787 with pMK315, which carries gldF; lanes 9 and 10, CJ787 with pMK321, which carries the gldFG region with an in-frame deletion in gldF; lanes 11 and 12, CJ288 (gldA mutant); and lanes 13 and 14, wild-type cells with pMK314. (B) Lanes 1 and 2, wild-type cells; lanes 3 and 4, CJ776 (Tn4351 insertion in gldG); lanes 5 and 6, CJ776 with pMK314; lanes 7 and 8, CJ776 with pMK315; lanes 9 and 10, CJ776 with pMK321; and lanes 11 and 12, CJ288 (gldA mutant).