FIG. 1.

(A) [³H]uridine incorporation into RNA after norvaline stress (0.05% [wt/vol]) in B. subtilis BR16 (wild type) and BR17 (relA). Control refers to RNA synthesis without norvaline addition (indicated by squares); RNA synthesis before and after norvaline addition is indicated by circles. The point of addition of norvaline (NV) is indicated by arrows. (B) Growth of B. subtilis BR16 (wild type) and BR17 (relA mutant) under control conditions (squares) and after norvaline addition (circles); solid symbols refer to BR16 (wild type), and open symbols refer to BR17 (relA mutant). (C) Percent incorporation of l-[³⁵S]methionine as measured in cultures grown with norvaline. B. subtilis BR16 (shaded columns) and BR17 (relA) (open columns) were compared.