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Sections of 2D protein gels (autoradiograms) of l-[35S]methionine-labeled proteins separated on immobilized pH gradient (IPG) strips in the pH ranges from 4 to 7 and from 4.5 to 5.5. Proteins were isolated from exponentially growing B. subtilis BR16 before (A) and 20 min after (B) norvaline addition and from B. subtilis BR17 20 min after norvaline addition (C). Only proteins induced (circles; arrows) or repressed (squares) independently of RelA are indicated.
