FIG. 8.

Northern blot analysis of the induction of the CodY-dependent gene gabP and the ure operon in response to the treatment of wild type and relA mutant with norvaline. Total RNA was isolated from strains BR16 and BR17 (relA) before (control [co]) and 10, 20, and 30 min after the addition of norvaline. A total of 5 μg of RNA was applied to each lane. The RNA probes gabP and ureA were used. The locations of RNA size markers, the size of the transcripts, and the transcriptional organization are indicated.