TABLE 1.
Site-directed mutants of C. crescentus ferrochelatase
| Mutation(s) | ΔhemH complementation | [2Fe-2S] cluster |
|---|---|---|
| H104A | + | + |
| T133C/A | + | + |
| S132A, T133A, T134A | − | Unstable |
| C158A | + | − |
| C328S | + | + |
| C332S | + | − |
| C339S | + | − |
| C341S | + | − |
| Δ328-347 | + | − |
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