TABLE 2.
Levels of β-galactosidase in transcription block strain when Par proteins were provided
| Strain and/or plasmid | par operona | Miller units
|
|
|---|---|---|---|
| Uninduced | Induced | ||
| CC4248 | None | 1 | 303 |
| CC4248 ihfA82::Tn10b | None | 7 | 454 |
| CC4248 pBR322 | None | 1 | 323 |
| CC4248 pALA2310 | parA+parB+ | 2 | 222 |
| CC4248 pALA2312 | parAM314I parB+ | 1 | 18 |
| CC4248 pALA1895 | ΔparA parB+ | 2 | 189 |
| CC4248 pALA2314 | parA+ ΔparB | 6 | 375 |
| CC4248 pALA2316 | parAM314I ΔparB | 8 | 377 |
a
Each par operon has a constitutive promoter and is present on the Cm resistance version of pBR322 (pALA1858; see Materials and Methods). Values are averages of at least three experiments.
b
Strain CC4249 is CC4248 with an additional ihfA82::Tn10 mutation.