TABLE 5.
Ability of transcription block mutations to support partition
| Resident plasmid | par operona | Cm-resistant colonies on overnight growthc | % Plasmid retention (25 generations) |
|---|---|---|---|
| pBR322 vector | None | ∼1 × 105 | 2 |
| pALA1570 | pcparA+parB+ | ∼1 × 105 | 92 |
| pALA1835 | pcparAM314I parB+ | <1 | NTd |
| pALA1863 | pcparAA14V parB+ | ∼5 × 101b | 18 |
| pALA1867 | pcparAD209Y parB+ | <1 | NT |
| pALA1888 | pcparAL329P parB+ | ∼5 × 101b | <1 |
| pALA1871 | pcparAD209G parB+ | <1 | NT |
| pALA1875 | pcparAD152N parB+ | <1 | NT |
| pALA1892 | pcparA+parBT12P | <1 | NT |
| pALA1841 | pcparA+ ΔparB | ∼1 × 105 | 1 |
a
Pc, Promoter constitutive.
b
No colonies were obtained after overnight growth, but small colonies were obtained after 48 hr; these were tested for plasmid retention. Values are averages of three experiments.
c
Number of lysogens of λ-P1:5RΔ1005::pALA1952 per assay. Each assay contained approximately 107 viable cells.
d
NT, no colonies were obtained for testing.