Fig. 4.

Activation of p38 MAP kinase and SAPK/JNK by CSE treatment. CSE induced the activation of p38 MAP kinase and SAPK/ JNK in HAECs. Treatments are listed on the top of the figure in the same condition as described in Fig. 3. A representative blot from three individual experiments is shown. Cell lysates were separated on a 10% SDS–PAGE. The membrane was immunoblotted with either anti-phospho p38 MAP kinase or anti-phospho SAPK/JNK antibodies. Pre-activation of eNOS by l-arginine prevented the CSE-induced kinase activation whereas inhibition of eNOS by l-NAME increased the CSE-induced activation. Total p38 MAP kinase and total SAPK/ JNK were unchanged.