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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: FEBS Lett. 2005 Jan 31;579(3):733–740. doi: 10.1016/j.febslet.2004.12.052

Fig. 5.

Fig. 5

Effects of p38 MAP kinase and/or SAPK/JNK inhibition on caspase-3 activation. Cells were pretreated with 10 μM of SB 202190, a p38 MAP kinase inhibitor, and/or SP600125, a JNK/SAPK inhibitor for 2 h as summarized on the top of the figure. This was followed by CSE (0.02 U) exposure for 4 h. Cell lysates were separated on 12% SDS–PAGE. The membrane was immunoblotted with anti-cleaved caspase-3 antibody. Inhibition of either p38 MAP kinase (lanes 3 and 4) or JNK/SAPK (lanes 5 and 6) resulted in partial reduction in CSE-induced caspase-3 activation. Furthermore, active caspase-3 returned to the control level when both p38 MAP kinase and SAPK/JNK were inhibited at the same time (lanes 7 and 8). Western blot with anti-actin was used as a loading control. Experiments were repeated three times and representative electrophoresis is shown in the figure.