Table 2.
Effect of Primary Smoking on eNOS and Its Regulatory Proteins
Nonsmokers | Primary smokers | |
---|---|---|
N | 84 | 5 |
eNOS protein, pg/μg protein | 14.0 ± 1.8 | 6.9 ± 2.4a |
eNOS activity, cpm/mg/min | 3350 ± 422 | 2294 ± 1761 |
eNOS mRNA, relative unit | 0.18 ± 0.04 | 0.31 ± 0.31 |
NOx, μM/μg protein | 0.311 ± 0.01 | 0.26 ± 0.05 |
Hsp90, relative intensity | 0.95 ± 0.15 | 0.53 ± 0.34 |
Caveolin-1, relative intensity | 0.95 ± 0.08 | 0.63 ± 0.15 |
Using analyses of variance, we tested the homogeneity in variance of the measured variables between smokers and nonsmokers. They were not significantly different for levels of eNOS protein and mRNA, NOx, Hsp90 and Caveolin-1.
P < 0.05 by independent Student t-test.