FIG. 7.

(A) Complementation of PAO-P10 with plasmid-based mvaT. The results shown are for PAO1 lecA::lux (⧫), PAO-P10 (□), PAO-P10 pUCP18 (•), and PAO-P10 pUCP mvaT (▵). (B) PA-IL lectin production in PAO1 and an mvaT chromosomal deletion mutant (PAO-P47) after 5, 7, 9, 11, 13, 15, and 25 h of growth. Shown is a Western blot of a P. aeruginosa cellular lysate after SDS-PAGE. The blot was developed with PA-IL antisera as described in Materials and Methods. PAO-P34 (rhlR mutant) was used as a PA-IL-negative control.