TABLE 1.
Plasmids used in this study
| Plasmid | Description | Reference or source |
|---|---|---|
| pUC19 | E. coli cloning vector; Apr, MCS | 26 |
| pGK14 | pGK13 (17) derivative lacking the Cmr gene; Emr | R. Breitling, unpublished |
| pRS10 | pBR322 replicon containing a translational repR-lacZ fusion; Ampr | 5 |
| pPR6 | pIP501 derivative containing a point mutation in the −35 box of promoter pIII | 6 |
| pMG25 | ColE1 derivative containing the lacI gene, an IPTG-inducible promoter, and the rrnB terminator; Ampr | M. Mikkelsen and K. Gerdes, unpublished |
| pGW380L | pSC101 derivative containing the promoterless lacZ gene with SDa sequence; Spcr | M. Lindell, unpublished |
| pGTC4 | pGK14-based vector containing the copR gene, pII/IR2, pIII, the attenuator, and 100 bp downstream | 9 |
| pUCpIII | pUC19 derivative containing the rnaIII gene with its own promoter | This study |
| pMGI | pMG25 derivative containing the promoterless pT181 rnaI gene | This study |
| pGTR | pGK14 derivative containing the rrnB terminator | This study |
| pGTR6 | pGTR derivative containing a pPR6-derived fragment upstream of the rrnB terminator | This study |
| pGTR181/35 | Like pGTR6, but with a fragment of pT181 carrying a point mutation in the −35 box of the RNAI promoter | This study |
| pGTR181/10 | Like pGTR6, but with a fragment of pT181 lacking the −10 box of the RNAI promoter | This study |
| pGW501 | pGW380L derivative containing a pPR6 sequence | This study |
| pGW181 | pGW380L derivative with a pGT181/35 sequence | This study |
| pGCZ6 | pGK14 derivative containing a pPR6 sequence fused in frame with the pRS10-derived lacZ gene | This study |
| pGT181/35 | pGK14 derivative with pGTR181/35 derived sequence | This study |
| pGKZ181 | pGT181/35 derivative carrying an in frame fusion with the pPRS10-derived lacZ gene | This study |
a
SD, Shine-Dalgarno sequence; MCS, multiple cloning site.