TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| S. mutans | ||
| NG8 | Wild type, Ems Kms | A. S. Bleiweis, University of Florida |
| BM71 | Wild type, Ems Kms | G. H. Bowden, University of Manitoba |
| SMCC1 | NG8::pComC-KO ComC− Emr Kms | 30 |
| SMCD1 | NG8::pComD-KO ComD− Emr Kms | 30 |
| SMCE1 | NG8::pComD-KO ComE− Emr Kms | 30 |
| SMComX1 | NG8::pComX-KO ComX1− Emr Kms | This study |
| SMCDE-L4 | NG8 ΔcomCDE::PcEm Emr Kms | This study |
| SMComX1 | NG8 ΔcomX1::PcEm Emr Kms | This study |
| SMCDE-L4-pCOMC | SMCDE-L4::pCOMC Spr Emr | This study |
| SMCC1-pCOMC | SMCDE-L4::pCOMC Spr Emr | This study |
| Plasmids | ||
| pDL289 | E. coli-Streptoccus shuttle vector, Kmr | 3 |
| pDL277 | E. coli-Streptococcus shuttle vector, Spr | 24 |
| pCOMC | PDL277 harboring a wild-type comC gene | This study |