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Effect of base substitutions on UhpA binding to uhpT promoter. DNase I protection assay was carried out on the uhpT promoter variants in the 180-bp DNA fragment extending from −140 to +40 and in the absence or presence of 800 nM UhpA. Nucleotide coordinates are listed on the left and were based on the position of A/G cleavage reactions (data not shown). The positions of the S and W regions are shown on the right. WT, wild type.
