FIG. 1.

Physical map of the chromosomal insertion sites of pGh9:ISS1. (A) Characterization of 18C3. The map of the pbp2b locus of Sfi6 was obtained from Stingele and Mollet (23). (B) Characterization of 6G4.The horizontal dashed lines represent the S. thermophilus genomic DNA. The large grey and black arrows represent the Erm^r gene and ISS1 elements of the plasmid, respectively. The thermosensitive replication origin of the plasmid is represented by small grey rectangles. Restriction sites: E, EcoRI; H, HindIII; and S, SalI. X-I^L and X-I^R (with X being the mutant name) indicate replicative molecules carrying the left and right junctions, respectively. The downward-pointing arrowheads indicate the site of pGh9:ISS1 insertion on the genome of S. thermophilus CNRZ368. The ORFs are indicated as open arrows. The filled flags indicate experimentally determined promoters (23), and the open flags indicate the putative promoters. The promoter of rodA is composed of a −35 sequence separated by 14 nucleotides from the extended −10 sequence. Putative rho-independent terminators are represented as hairpin loops.