FIG. 2.

Northern analysis of rodA from S. thermophilus CNRZ368. (A) Schematic showing the location of the rodA RNA probe utilized in the Northern experiment. The downward-pointing arrowhead indicates the site of pGh9:ISS1 insertion, and the open flag indicates the putative promoter. (B) Northern blot analysis of orf1 transcript. Lane 1, Results of ethidium bromide staining of RNA (30 μg) from S. thermophilus CNRZ368 after agarose gel electrophoresis into a formaldehyde-denaturing (0.8%) agarose gel. RNAs were extracted from late-exponential-phase (OD₆₀₀ of 1.4) culture in TPPY medium. 16S and 23S indicate the migrating positions of 16S and 23S rRNA, respectively. Lane 2, Result of the hybridization with the rodA probe. nt, nucleotides.