FIG. 2.

The RcsCB system is necessary to overcome inhibition of growth by CPZ. Cultures of strains CLG686 [Φ(osmCp₁-lacZ) rcsB⁺ rcsC⁺; diamond), CLG698 (CLG686 rcsB::tet; square), and CLG768 (CLG686 rcsC::tet; circle) in LB0N broth grown overnight were diluted 1,000-fold in the same medium and grown to an OD₆₀₀ of ∼0.1 at 37°C. At time zero, CPZ was added to a final concentration of 100 μM, and the OD₆₀₀ (A) and the β-galactosidase specific activity (B) were determined at the indicated times. β-Galactosidase activity is expressed in Miller units. Cultures were treated with CPZ (closed symbols) or were not treated (controls) (open symbols).