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UDP-GlcNAc 4-epimerase activity in cell extracts of E. coli strains
| Strain | GlcNAca (μM) | Sp actb [U (mg of protein)−1] | % of wild-type activity |
|---|---|---|---|
| M1685 | 1.822 | 0.0048 | 100 |
| M2313 | 0 | 0 | 0 |
| M2318 | 2.151 | 0.0057 | 118 |
Concentration of UDP-GlcNAc after the reaction.
One unit of enzyme is defined as the amount which catalyzes the formation of 1 μmol of UDP-GlcNAc in 1 min under the conditions of our assay.
