TABLE 2.
PDH activity of the wild type and pdh mutants
| Strain | Relevant genotype | PDH activitya |
|---|---|---|
| JH642 | pdh+ | 0.111 ± 0.002 |
| 501-76 | ΔpdhB | 0.000 ± 0.002 |
| 501-77 | ΔpdhC | 0.000 ± 0.002 |
| 501-78 | ΔpdhD | 0.000 ± 0.002 |
| 501-76 + 501-77b | ΔpdhB + ΔpdhC | 0.013 ± 0.002 |
| 501-77 + 501-78b | ΔpdhC + ΔpdhD | 0.009 ± 0.002 |
Change in absorbance at 366 nm per minute per milligram of protein. Values are means ± standard errors.
Mixture of equal amounts (protein concentration) of cell extract from each mutant.