TABLE 3.
Sporulation of the wild type and pdh mutants in NSM
| Strain | Relevant genotype | Maximum titer (108)a | Titer (108) at t20b
|
Sporulation efficiencyc | Relative sporulation frequencyd | |
|---|---|---|---|---|---|---|
| Cell | Spore | |||||
| JH642 | pdh+ | 6.1 | 4.9 | 4.1 | 0.67 | 1.00 |
| 501-76 | ΔpdhB | 2.1 | 0.8 | 0.05 | 0.03 | 0.05 |
| 501-77 | ΔpdhC | 1.9 | 0.7 | 0.05 | 0.03 | 0.05 |
| 501-78 | ΔpdhD | 3.8 | 2.9 | 1.4 | 0.38 | 0.54 |
a
Concentration of viable cells at the highest cell density.
b
The time of onset of stationary phase is designated t0. The sporulation frequency of each strain at t20 was almost the same as the maximum frequency (<5%).
c
Ratio of spore titer to maximum titer.
d
Ratio of the sporulation frequency of each strain to that of the wild type.