Skip to main content
. 2002 May;184(10):2595–2602. doi: 10.1128/JB.184.10.2595-2602.2002

FIG. 7.

FIG. 7.

Degradation of β-casein by the purified YibP-His6 fusion protein. The standard reaction mixture was described in Materials and Methods. Proteins of the gel were stained with CBB. (A) Lane 1, molecular size markers. Lane 2, without YibP-His6. Lane 3, addition of 1 mM ATP and 25 μM zinc acetate. Lane 4, standard reaction mixture (2 μg of β-casein). Lane 5, without β-casein. All the samples (lanes 2 to 5) were incubated for 10 h at 37°C. (B) Lane 1, molecular size makers. Lane 2, without YibP-His6 (time zero). Lane 3, without YibP-His6. Lane 4, standard reaction mixture. Lane 5, without YibP-His6 and with the supernatant of the YibP-His6 sample treated with anti-His tag mouse monoclonal IgG1 and protein A-Sepharose. Reaction mixtures (lanes 3 to 6) were incubated for 10 h at 37°C. (C) Time course of the reaction. Solid circles, with YibP. Open circles, without YibP. (D) Reaction mixtures containing 4 μg of β-casein were incubated for 10 h at 37°C. Lane 1, molecular size markers. Lane 2, β-casein. Lane 3, standard reaction mixture (4 μg of β-casein). Lane 4, without magnesium acetate. Lane 5, without magnesium acetate and with 10 mM EDTA.