TABLE 2.
Plasmids used
| Plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| pHSG396 | Cmr; cloning vector | Takara |
| pACYC177 | Kmr; ColE1 cloning vector | 3 |
| pKH5002 | Apr; Δori rpsL+ | 17 |
| pHSG415s | Cmr; temperature-sensitive plasmid | 5 |
| pET21b | Apr; T7 promoter vector | Novagen |
| pACYC184 | Cmr Tcr; ColE1 cloning vector | 3 |
| pIT101 | Cmr; yibP cloned at BamHI and KpnI sits of pHSG396 | This study |
| pIT102 | Cmr Kmr; kan cassette inserted at Aor51HI sites of pIT101 | This study |
| pIT201 | Apr Kmr; BamHI-KpnI yibP::kan segment inserted at BamHI and KpnI sites of pKH5002 | This study |
| pHSGY | Cmr; yibP fragment inserted at PstI and SmaI sites of pHSG415s | This study |
| pETY | Apr; yibP fragment inserted at NdeI and XhoI sites of pET21b | This study |
| pIT401 | Cmr; TnphoA inserted in yibP of pIT101 | This study |
| pIT402 | Cmr; TnphoA inserted in yibP of pIT101 | This study |