TABLE 2.
Summary of proteome analysis, Northern hybridization analysis, and effect of protease inhibitors on the two-dimensional pattern
| Spot no. | CPE no. | Product (homologous protein) (reference) | Patterna | Densityb
|
Northernc | Effect of protease inhibitord
|
||
|---|---|---|---|---|---|---|---|---|
| Wild type | Mutant | L, A | B | |||||
| P1 | CPE1951 | 2′,3′-cyclic nucleotide 2′-phosphodiesterase | W | 726 | 474 | P (0.26) | +/− | +/− |
| P2 | CPE0163 | Perfringolysin O | W | 22,167 | 732 | P (0.22) | +/− | − |
| P3 | CPE1529 | Hypothetical protein (ydaL gene product from Bacillus subtilis [29.3%]) (17) | W | 2,017 | 0 | P (0.83) | +/− | +/− |
| P4 | CPE0846 | Cysteine protease (alpha-clostripain from Clostridium histolyticum [58.3%]) (7) | W | N.D. | N.D. | P (0.32) | +/− | − |
| P5 | CPE0173 | Collagenase | W | 530 | 0 | P (0.30) | +/− | +/− |
| P6 | CPE1231 | Probable surface protein (Aas surface protein from Staphylococcus saprophyticus [25.4%]) (13) | W | 11,243 | 0 | I (1.09) | − | − |
| P7 | CPE0220 | Hypothetical protein (chitinase A from Clostridium paraputrificum [38%]) (24) | W | 1,158 | 0 | I (1.04) | +/− | +/− |
| N1 | CPE1428 | ClpB protein (endopeptidase Clp ATP-binding chain B1 from Synechocystis sp. [55.8%]) (15) | M | 0 | 4,780 | I (1.00) | + | + |
| N2 | CPE0202 | Probable cell wall-binding protein (cell wall-binding protein from Bacillus halodurans [32.4%]) (31) | M | 0 | 3,839 | N (1.51) | + | + |
| N3 | CPE1785 | Iron-sulfur cofactor synthesis protein NifS | M | 3,015 | 13,371 | I (1.05) | + | + |
| N4 | CPE1632 | Ribokinase | M | 0 | 5,352 | I (1.08) | + | + |
| N5 | CPE2297 | β-Hydroxybutyryl-coenzyme A dehydrogenase, NAD dependent | M | 0 | 6,403 | I (1.00) | +/− | +/− |
| N6 | CPE1350 | Fructose-bisphosphate aldolase | M | 0 | 10,109 | N (1.46) | + | + |
| N7 | CPE2408 | Elongation factor G | M | 1,792 | 3,525 | I (0.95) | +/− | +/− |
| N8 | CPE0278 | Conserved hypothetical protein (p45 from Listeria monocytogenes [26%]) (26) | M | 0 | 8,698 | I (0.98) | +/− | +/− |
a
W, exclusive to the wild type, M, exclusive to the virR mutant.
b
Values represent the ratio of spot density level (in ppm) to the total of all valid spots using PDQuest software (Bio-Rad). N.D., not determined.
c
P, positively regulated; I, independently regulated; N, negatively regulated. Values represent the ratio of specific mRNA level in the mutant to that in the wild type.
d
+/−, not altered; +, induced; −, reduced. L, leupeptin; A, antipain; B, benzamidine.