TABLE 3.

Effect of inhibitors on proteolytic activity^a

Inhibitor	Concn	% Inhibition
		Supernatant	Purified
PMSF	0.5 mM	1	21
Leupeptin	10 μM	84	100
Antipain	10 μM	82	100
E-64	0.7 μM	0	16
Pepstatin	1 μM	0	14
EDTA	10 mM	82	73
Benzamidine	5 mM	74	87
SBTI	0.1 mg/ml	0	17
Phosphoramidon	20 μM	0	16

^aProteolytic activities of the culture supernatant of wild-type and purified protease were determined with azocoll in the presence of inhibitors. Activities are expressed as percentages of the respective controls. E-64, l-trans-epoxysuccinyl-leucylamido(4-guanidino)butane; SBTI, soybean trypsin inhibitor.