TABLE 2.
List of ORFs of plasmid pHel5
| ORF | Codon positions (start-stop) | Sequencea | Molecular mass (kDa) | pI | Net charge | Propertiesb | Homology (BLASTP) |
|---|---|---|---|---|---|---|---|
| orf5A | 865-2481 | CCAAAAGATAAGGAGTATAGAGTG | 47.0 | 10.25 | 19 | cc; mix | RepA, 0.0 |
| orf5B | 3352-2706 | GCAACTTACACAGGAAAAACAATG | 25.2 | 10.36 | 13 | SP; mix | JHP1295, 2e-37; HP1382, 2e-05 |
| orf5C | 3761-4279 | AAAGGAAAGGAAAATGCAACCATG | 19.9 | 7.81 | 1 | α | |
| orf5D | 4898-5749 | CAACACAAGGAGATTTTAAAAATG | 34.1 | 9.73 | 7 | cc; α | |
| orf5E | 5862-6929 | CACAAGCAAGAAGGACCAAAAATG | 41.6 | 9.28 | 7 | Mix | |
| orf5F | 7387-7121 | ATGAACTAAAAAGAGAGATACATG | 10.8 | 9.24 | 3 | Mix | HP0894, 4e-22; JHP831, 2e-17; HP0892, 3e-17; JHP0825, 5e-13 |
| orf5G | 7652-7374 | AGCTTAATACAAGGAAATGAGATG | 10.8 | 10.19 | 5 | cc; | JHP0828, 4e-11; HP0316, 0.030 |
| orf5H | 8271-8783 | ACTATTTTTATCAATGTCGCAATG | 19.8 | 8.49 | 3 | cc; α | |
| orf5I | 8795-9196 | AGAAGAATAGGAACAAAAAGAATG | 15.0 | 8.50 | 3 | SP; cc; α-β | |
| orf5J | 9193-9405 | GAAAGAACAATTGGATGACTTATG | 8.1 | 9.99 | 4 | Mix | |
| orf5K | 9440-10048 | TTAGACACTAGGAACAAAGTGATG | 23.4 | 5.60 | −10 | Mix | JHP0651, 3e-31; HP0712, 2e-22 |
| orf5L | 10800-11474 | CATTAAATATAAAGGAACAGAATG | 25.5 | 5.96 | −2 | Mix | HP1000, 4e-53; JHP0935, 1e-36 |
| orf5M | 11471-12037 | ACCCAACGAAGAAAGGAAAGTATG | 21.7 | 9.76 | 3 | cc; mix | |
| orf5N | 12898-13491 | CACATAAAAAGGATAAAAATTATG | 23.2 | 9.18 | 5 | Mix | HP0879, 7e-08; JHP812, 8e-08; HP1334, 2e-06 |
| orf5O | 13571-14731 | ATTTTTCCACAAAGGATCGCAATG | 46.7 | 8.98 | 5 | cc; mix | |
| orf5P | 14834-15085 | AAATTTACTAGGAATAGTAAAATG | 9.8 | 4.67 | −4 | Mix | HP0993, 7e-30 |
| orf5Q | 15057-15769 | TCCCTATGGAAAATATTCAGTATG | 27.8 | 6.52 | −4 | Mix | HP0994, 2e-94 |
a
The putative Shine-Dalgarno sequence is underlined; the start codon is in boldface.
b
TM, transmembrane domain; SP, signal peptide; α, α-β, and mix, secondary structure elements as predicted by PREDICTPROTEIN; cc, coiled-coil structure.