FIG. 4.

Effect of S-motility rescue on developmental cheating in mixed culture. Solid columns indicate spore counts for the ancestor, and open columns give the counts for unrescued S2/pDW79/− (a) and rescued S2/pDW79/+ (b). Dashed lines in the open columns are the expected sporulation levels for a neutrally marked wild-type strain at each initial frequency. Spore counts for evolved clone variants that exceed the expected level indicate developmental cheating (40). Error bars indicate 95% confidence intervals. The short column with no error bars indicates a sporulation level below the level of detection, which is shown by the column height.