FIG. 1.

The N-terminally modified forms of TraG, TraD, and HP0524 were solubilized and purified as described in Materials and Methods. Purification steps and yields are listed in Table 4. For each protein 7 μg was loaded onto an SDS-15% polyacrylamide gel, resolved by electrophoresis, and stained with Coomassie blue R. Lane 1, marker proteins; lane 2, purified TraG (His₆-TraG); lane 3, purified TraD (His₆-TraD); lane 4, purified HP0524Δ1 (His₆-HP0524Δ1).