TABLE 2.
Plasmids and phages used in this study
| Plasmid | Description | Relevant genotype | Selective marker(s)a | Reference or source |
|---|---|---|---|---|
| M13mJF182 | M13mp18Ω(pJF142 BamHI-BamHI, 0.8-kb fragment) | oriT | 63 | |
| pBS140 | pJF119HEΔ(HincII-SphI)Ω(RP4 48710-46495b) | traG+ | Ap | 61 |
| pDB127 | pDB126Δ(RP4 SfiI-SspI 48374-46670) | (trbB-trbM)+(traF-traM)+traG0 | Cm | 4 |
| pET14b | Vector for production of His6 fusion proteins | Ap | Novagen | |
| pFS141 | pMS470Δ8Δ(NdeI-HindIII)Ω(RP4 48495-46588 his6c) | traG-his6+ | Ap | This work |
| pFS241 | pMS470Δ8Δ(NdeI-HindIII)Ω(his6 RP4 48495-46588) | his6-traG+ | Ap | This work |
| pHY524Δ1 | pET14bΔ(NdeI-BamHI)Ω(Hp 550217-551951d) | his6-hp0524Δ1+ | Ap | This work |
| pJF142 | pBR329Ω(BamHI, BamHI-XmaIII linker, RP4 XmaIII-XmaIII 50995-51770, XmaIII-BamHI linker) | oriT | Ap, Cm | 20 |
| pJF143 | pBR329Ω(BamHI, BamHI-XmaIII linker, RP4 XmaIII-AccI 50995-51269, AccI-BamHI linker) | oriT | Ap, Cm | 20 |
| pKI410 | HpaI-BamHI fragment from pMP1 in pSPORT | traD+ | Ap | 33 |
| pMS119EH | Vector, Plac/lacIq | Ap | 53 | |
| pMS470Δ8 | Vector, pMS119EHΔ(XbaI-PstI)Ω(pT7-7 XbaI-NdeI, 40-bp fragment, R751 traC AvaI-SphI, 1.4 kb), Plac/lacIq | traC+ | Ap | 5 |
| pOX38traD411 | In vivo recombinant of pOX38 × pK1411 | traD0 | Km | 33 |
| pSK410 | pMS470Δ8Δ(NdeI-HindIII)Ω(F 23779-25937e) | traD+ | Ap | This work |
| pSK410CH | pMS470Δ8Δ(NdeI-HindIII)Ω(F 23779-25937 his6) | traD-his6+ | Ap | This work |
| pSK410NH | pMS470Δ8Δ(NdeI-HindIII)Ω(his6 F 23779-25937) | his6-traD+ | Ap | This work |
| pSK470 | pMS470Δ8Δ(NdeI-HindIII)Ω(RP4 48495-46588) | traG+ | Ap | This work |
| pSK470ΔB | pSK470Δ(BamHI)Ω(GATC) | traG+ | Ap | This work |
a
Ap, ampicillin; Cm, chloramphenicol; Km, kanamycin.
c
his6 sequence: CACCATCACCATCACCAT.