TABLE 3.
Transfer activity of modified TraG and TraD proteins
| Donor strain (transfer deficient)a | Recipient straina | Plasmid tested for complementation | Description | Transfer rateb |
|---|---|---|---|---|
| HB101(pDB127) (Cm) | HB101 Nxr (Nx) | pFS241 | his6-traG | 5.0 × 10−1 |
| pFS141 | traG-his6 | 3.0 × 10−1 | ||
| pSK470 | wt traG (overproducer) | 3.0 × 10−1 | ||
| pBS140 | wt traG | 5.0 × 10−1 | ||
| pMS119EH | Vector alone | <1.0 × 10−6 | ||
| XK1200(pOX38traD411) (Km) | SG13109 (Tc) | pSK410NH | his6-traD | 3.3 × 100 |
| pSK410CH | traD-his6 | 2.0 × 10−3 | ||
| pSK410 | wt traD (overproducer) | 7.6 × 100 | ||
| pK1410 | wt traD | 3.7 × 100 | ||
| pMS119EH | Vector alone | <1.0 × 10−7 |
a
Antibiotic resistances are in parentheses. Nx, nalidixic acid; Tc, tetracycline.
b
The transfer rate is given by the number of transconjugants per donor cell. Transconjugants were selected for expression of Cm and Nx (HB101 Nxr) resistance or Km and Tet (SG13109) resistance.