TABLE 6.

Transfer activity and trypsin sensitivity of TraG insertion mutants

traG plasmid	TraG protein	No. of transconjugants/donor	Transfer activitya	Trypsin sensitivityb
pSK470	TraG	40	++	−
pMS119	None	<10−6	−	ND
ptraG::i31Ser30	TraGiS30	4 × 10−4	+/−	ND
ptraG::i31Gly32	TraGiG32	l × 10−4	+/−	ND
ptraG::i31Gly53	TraGiG53	10	++	+
ptraG::i31Gly74	TraGiG74	2 × 10−2	+	+
ptraG::i31Gly87	TraGiG87	8 × 10−5	+/−	+
ptraG::i31Ala119	TraGiA119	1 × 10−3	+	−
ptraG::i31Glu141	TraGiE141	3	++	ND
ptraG::i31Ser153	TraGiS153	2	++	−
ptraG::i31Glu175	TraGiE175	4 × 10−3	+	ND
ptraG::i31Asn243	TraGiN243	7 × 10−1	++	−
ptraG::i31Glu255	TraGiE255	4 × 10−2	+	ND
ptraG::i31Ala344	TraGiA344	5 × 10−3	+	−
ptraG::i31Ser397	TraGiS397	74	++	−
ptraG::i31Gly455	TraGiG455	<10−6	−	ND
ptraG::i31Lys473	TraGiK473	<10−6	−	ND
ptraG::i31Asn482	TraGiN482	6 × 10−5	+/−	−
ptraG::i31Glu516	TraGiE516	2 × 10−4	+/−	−
ptraG::i31His517	TraGiH517	<10−6	−	ND
ptraG::i31Glu552	TraGiE552	<10−6	−	ND
ptraG::i31Val612	TraGiV612	61	++	−

^aTransfer activity is defined as positive (++) with wt TraG (pSK470) or negative (−) without TraG (pMS119) expressed from the complementary plasmid in the mating experiment. + and +/−, intermediate and weak activities, respectively.

^bND, not determined.