Yeast growth assays in the presence of 2-MeSADP, ADP, ATP. Representative data exhibiting the concentration-dependent growth (represented as OD630) of the engineered yeast strain expressing P2Y1 receptors in the presence of 2-MeSADP, ADP, and ATP ranging in concentration from 10−10 to 10−3 (M). The EC50 values averaged over at least three experiments are 2-MeSADP (130 nM) > ADP (1800 nM) > ATP (28,000 nM). Experiments were performed at least three times with representative data shown. Within each experiment each data point was done in triplicate and averaged.