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Yeast growth assay in the presence of the antagonist MRS2179. MRS2179 (N6-methyl-2-deoxyadenosine-3′,5′-bisphosphate) was used to antagonize ADP (adenosine 5′-diphosphate) via a yeast growth assay (represented as OD630). The MRS2179 concentration was fixed at either 0, 10−6, 10−5, or 10−4 (M) over an ADP range of 10−10 to 10−4 M. Increasing amounts of antagonist (10−6, 10−5, 10−4 M) cause a right shift in the curves. Each experimental data point was done in triplicate and averaged.
