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(Left) Mobility shift of the ccaR-234 probe. Lane 1, free probe; lane 2, assay mixture with 1 μg of purified CcaR; lane 3, assay mixture with 1.5 μg of purified CcaR. (Right) Mobility shift of the lat-181 probe. Lane 1, free probe; lane 2, assay mixture with 1 μg of purified CcaR; lane 3, assay mixture with 1.5 μg of purified CcaR.
