TABLE 2.
Quantitation of cell length and FtsZ rings after MinC/DicB-mediated filamentationa
| Plasmid | Time (min) | R− cells
|
R+ cells
|
R− + R+ cells
|
||||
|---|---|---|---|---|---|---|---|---|
| % (n) | Avg length (range) (μm) | % (n) | Avg length (range) (μm) | L/R (μm) | Avg length (range) (μm) | L/R (μm) | ||
| pJE44 [PBAD::dicB] | 10 | 20 (9) | 6.7 (3.2-20.0) | 80 (36) | 13.4 (4.4-33.5) | 5.5 | 12.0 (3.2-33.5) | 6.1 |
| 60 | 7 (2) | 21.9 (8.0-35.8) | 93 (25) | 15.1 (4.2-49.8) | 4.9 | 15.6 (4.2-49.8) | 5.4 | |
| 120 | 35 (9) | 39.0 (4.2-66.3) | 65 (17) | 30.3 (2.6-70.8) | 16.1 | 33.3 (2.6-70.8) | 27.1 | |
| 180 | 30 (17) | 51.4 (3.5-98.4) | 70 (39) | 11.0 (2.5-97.6) | 8.6 | 23.3 (2.5-98.4) | 26.0 | |
| pBAD33 (vector) | 0 | 16 (14) | 2.5 (1.9-3.5) | 84 (72) | 3.5 (1.9-15.9) | 2.7 | 3.4 (1.9-15.9) | 3.1 |
| 180 | 22 (13) | 2.5 (1.8-3.8) | 78 (47) | 2.8 (1.9-4.5) | 2.7 | 2.7 (1.8-4.5) | 3.4 | |
a
Cells of strain PB147(λDR120) [ΔminDE(Plac::gfp-ftsZ)] harboring the indicated plasmid or vector were treated and analyzed as described in the text. Parameters were calculated separately for cells without fluorescent ring structures (R− cells), cells with one or more rings (R+ cells), and all cells combined (R− + R+ cells). n, number of cells examined. Any visible accumulation of fluorescence resembling a ring was counted as one.