TABLE 1.

Effect of traV cysteine mutations on biological functions

Plasmid(s)	Phage titer (PFU/ml)a		No. of Kanr reductants/cell by M13K07c	No. of Camr Tetr transconjugants cell for DNA donor activityd
	R17	M13K07
pOX38 traV::cat	<105	<105	<1.2 × 10−7	<1 × 10−6
+ pUC traV	1 × 1011	1 × 1011	0.21	1.0
+ pUC traVC10S	0.4 × 1011	1 × 1011	0.41	0.22
+ pUC traVC18S	NDb	ND	0.014	0.14
+ pUC traVC10/18S	0.3 × 1011	0.5 × 1011	6.8 × 10−4	0.09

^aTiters on RD17/pOX38 tra⁺ cells were 1 × 10¹¹ PFU/ml for R17 and 1.3 × 10¹¹ PFU/ml for M13K07.

^bND, not determined (see text).

^cMeasured as described in reference 1.

^dThe recipient strain was AE2248 (F⁻ thr-34::Tn10).