TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotype and/or description | Source or reference |
|---|---|---|
| Strains | ||
| Photorhabdus K122 primary | Wild isolate from Ireland | 22 |
| Photorhabdus K122 secondary | Isolated from prolonged stationary-phase culture of primary cells | 41 |
| Photorhabdus KN1 | Primary K122 with ner disrupted | This study |
| Photorhabdus KN2 | Secondary K122 with ner disrupted | This study |
| E. coli Tg-1 | supE44 hsdΔ5 thi-1 Δ(lac-proAB) F′(traD36 proAB+lacIqlacZΔM15) | Stratagene |
| E. coli XL1-Blue | F′ proAB lacIqZΔM15 Tn10(Tetr) | Stratagene |
| Plasmids | ||
| pBR322 | Apr Tetr with BamHI site, 4.4 kb | Promega Corporation |
| pGEM-7Zf(−) | Apr, multiple cloning site, 3 kb | Promega Corporation |
| pHP45Ω-Km | Source of Kmr interposon | 12 |
| pBS115 | Source of sacB gene; same as pBS101 (4) except sacB/sacR not under control of the lac promoter | Todd Ciche |
| pK2B | 1.5-kb insert containing ner in BamHI site of pBR322 | This study |
| pK2N101 | 1.2-kb ner-containing insert from pK2B in EcoRI site of pGEM7Zf(−) | This study |
| pK2N102 | 0.85-kb insert (without ner) from pK2B between EcoRI and SphI sites in pGEM7Zf(−) | This study |
| pDR100 | pK2B with ner disrupted by Kmr interposon from pHP45Ω-Km | This study |
| pDR101 | pDR100 with sacB gene from pBS115 in EcoRV site | This study |