TABLE 2.
Phenotypes of phase 1 cells transformed with ner-containing plasmid pK2N101 (white colonies)
| Phenotype | Primary | Secondary | Primary/pK2N101 (white transformants) |
|---|---|---|---|
| Pigment (on LB) | Yellow | White | White |
| Colony consistency | Mucoid | Nonmucoid | Nonmucoid |
| Bioluminescence | +++ | − | − |
| Lipase | +++ | − | − |
| Protease | +++ (6.2)a | − | + (2.4)a |
| Antibiotics | +++ | − | − |
| Crystal proteins | + | − | − |
| Dye uptake | |||
| NBTA | Green | Red | Red |
| MCA | Red | Pink | Pink |
| EBA | Black | Purple | Purple |
| MCBPA | Green | Purple | Purple |
| Congo Red | ++ | − | − |
| Hemolysin | ++ | − | − |
a
Values represent units of protease per milliliter of supernatant from cultures grown to an OD600 of 3.