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Western immunoblot analysis of M. pneumoniae with anti-HMW3 serum. Equal amounts of total protein were electrophoresed on an SDS-4.5% polyacrylamide gel, transferred to nitrocellulose, and probed with antisera (1:10,000). Lanes: 1, wild-type M. pneumoniae; 2, hmw3::Tn4001 transformant; 3, transformant control (modified Tn4001 transposon inserted elsewhere). Protein size standards are indicated in kilodaltons.
