FIG. 3.

Western immunoblot analysis of M. pneumoniae to assess steady-state levels of certain cytadherence-associated proteins. Equal amounts of protein were electrophoresed on either a 4.5% (A, B, and D) or a 10% (C and E) polyacrylamide gel, transferred to nitrocellulose, and probed with the following antisera: A, anti-HMW1; B, anti-HMW2; C, anti-P30; D, anti-B; E, anti-P65. Lanes: 1, wild-type M. pneumoniae; 2, hmw3::Tn4001 transformant; 3, transformant control; 4 (panel E only), mutant I-2. Protein size markers are given in kilodaltons to the left of each blot, and arrowheads designate proteins of interest.