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Western immunoblot analysis of M. pneumoniae to compare levels of protein P43. Equal amounts of protein were electrophoresed on a 12% polyacrylamide gel, transferred to nitrocellulose, and probed with antisera (1:100). Lanes: 1, wild-type M. pneumoniae; 2, hmw3::Tn4001 transformant. The protein size standard is indicated in kilodaltons.
