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RT-PCR analysis of RL101. Lanes 1 to 4, amplification of a ∼350-bp fragment upstream of the Ermr insertion site using primer pair BamCheAF and JSCheA; lanes 5 to 8, amplification of an ∼260-bp fragment downstream of the Ermr insertion site using primer pair BamHIF and X-BglR. Lanes 1, 2, 5, and 6, wild-type samples; lanes 3, 4, 7, and 8, cheA mutant RNA. Samples in lanes 2, 4, 6, and 8 were treated with reverse transcriptase, whereas samples in lanes 1, 3, 5, and 7 were not.
