FIG. 9.

Salicylate enhances PhlF binding to phO. Binding reaction mixtures containing 2 ng of radiolabeled 7EH probe, 3 μg of PhlF-6xHis, and increasing amounts of PHL were prepared with or without 4 mM salicylate. Binding was measured by densitometry, and the relative PhlF binding activity in the presence and absence of salicylate was calculated for each concentration of PHL. This is presented as the natural log of the intensity of bound probe divided by the total intensity of the probe. Sal, salicylate.