TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| Pseudomonas fluorescens F113 | Wild-type; HCM+ Prt+ Lac− | 55 |
| Escherichia coli DH5α | φ80 lacZΔM15 Δ(lacZYA-argF)U169 hsdR17 recA1 endA1 thi-1 | 52 |
| Plasmids | ||
| pMP190 | Promoterless lacZ vector, IncQ Cmr Smr | 57 |
| pMP220 | Promoterless lacZ vector, IncP Tcr | 57 |
| pQE-60.27 | 700-bp NcoI-BglII fragment containing PCR-amplified phlF in pQE-60 | 12 |
| pCU107 | phACBD-lacZ, 5-kb EcoRI fragment from pCU203 in pMP220, Tcr | 12 |
| pCU109 | phlF-lacZ, 1.8-kb SalI-KpnI fragment from pCU203 in pMP190, Cmr | 12 |