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. 2002 Jun;184(11):2914–2924. doi: 10.1128/JB.184.11.2914-2924.2002

FIG. 2.

FIG. 2.

(A) Analysis by SDS-PAGE of the CBP-HbpR protein fraction after the single calmodulin resin purification step. Lanes: L, protein markers (sizes given in kilodaltons); 1, protein fraction purified from E. coli BL21(pHB150), expressing CBP-HbpRΔ; 2, protein fraction purified from E. coli BL21(pHB151), expressing CBP-HbpR (67 kDa). The contaminating proteins have sizes of 60 and 75 kDa. (B) Expression of luciferase activity from the hbpC promoter of P. azelaica reproduced in E. coli harboring plasmid pHB164 (black bars) and incubated with 25 μM 2-HBP. Plasmid pHB164 contains the cbp-hbpR fusion gene, the hbpC promoter, and the luxAB genes. As a negative control, E. coli harboring plasmid pHB165 (white bars) was used. pHB165 is identical to pHB164, except for a frameshift in hbpR. As a positive control, E. coli harboring plasmid pHB208 was used (grey bars). pHB208 is identical to pHB164, except that it contains the native hbpR gene. Luciferase activity was measured after 2 h of induction at 37°C. RLU, relative light units.