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. 2002 Jun;184(11):2914–2924. doi: 10.1128/JB.184.11.2914-2924.2002

FIG. 7.

FIG. 7.

GMSAs with fragments containing modified UASs C-1 and C-2 and CBP-HbpR. (A) Effect of deleting one UAS on the binding of CBP-HbpR. Lanes: A, incubation of DNA fragment hbpCCC6, containing the native configuration of UASs C-1 and C-2, with (+) or without (−) 600 nM CBP-HbpR; B, incubation with the insert of pHB156 containing only the proximal UAS, C-1; C, incubation with the insert of pHB157 containing only the distal UAS, C-2 (12). (B) Effect of adding 5 and 10 bp between the two palindromes comprising UASs C-1 and C-2. Lanes: A, incubation with fragment hbpCCC6; B, incubation with the insert of pHB163, containing the 10-bp insertion; C, incubation with the insert of pHB162, containing the 5-bp insertion. The amount of CBP-HbpR added to each binding assay mixture is indicated above each lane.