TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or characteristics | Source or reference |
|---|---|---|
| E. coli strains | ||
| DH5α | endA1 hsdR17 (rK− mK−) supE44 thi-1 recA1 gyrA96 relA1 F− Δ(argF-lacZYA)U169 (φ80dlacZΔM15)λ− | Gibco BRL |
| BL21(DE3)(pLysS) | ompT lon hsdSB (rB− mB−) gal dcm (DE3) pLysS Cmr | Stratagene |
| Plasmids | ||
| pGEM-T-Easy | Apr | Promega |
| pCAL-n-FLAG | Apr; expression vector | Stratagene |
| pHBP130 | Apr ColE1; contains a 7.8-kb MluI-SalI fragment from P. azelaica HBP1 with hbpR and hpbC | (29) |
| pHYBP109 | Apr ColE1; pJAMA8 carrying hbpR under the control of its native promoter (PhbpR) | (13) |
| pHYBP132 | Apr ColE1; pET3d containing hbpR under the control of the φ10 promoter | (13) |
| pJAMA8 | Apr ColE1; luxAB-based promoter-probe vector | (13) |
| pHB150 | Apr; pCAL-n-EK vector Stratagene carrying the 0.5-kb PCR fragment containing the start of hbpR | This study |
| pHB151 | Apr; pHB150 carrying the 1.5-kb NsiI-SalI fragment from pHYBP132 which generated a complete hbpR | This study |
| pHB152 | pGEM-T-Easy containing a 117-bp fragment with UAS C-2 obtained by PCR with primers hbpC11 and hbpC12 | This study |
| pHB153 | pGEM-T-Easy containing an 85-bp fragment obtained by PCR with primers hbpC11 and hbpC15 | This study |
| pHB154 | pGEM-T-Easy containing an 86-bp fragment obtained by PCR with primers hbpC10 and hbpC13 | This study |
| pHB155 | pGEM-T-Easy containing a 118-bp fragment with UAS C-1 obtained by PCR with primers hbpC10 and hbpC14 | This study |
| pHB156 | pHB152 containing a 450-bp NaeI-BamHI fragment from pHB154; contains only UAS C-2 | This study |
| pHB157 | pHB153 containing a 480-bp NaeI-BamHI fragment from pHB155; contains only UAS C-1 | This study |
| pHB158 | pGEM-T-Easy containing a 118-bp fragment with UAS C-2 obtained by PCR with primers hbpC11 and hbpC16 | This study |
| pHB160 | pGEM-T-Easy containing a 110-bp fragment with UAS C-1 obtained by PCR with primers hbpC10 and hbpC17 | This study |
| pHB161 | pGEM-T-Easy containing a 115-bp fragment with UAS C-1 obtained by PCR with primers hbpC10 and hbpC18 | This study |
| pHB162 | pHB158 containing the 470-bp NaeI-BamHI fragment from pHB160 with UAS C-1; contains UASs C-1 and C-2 + 5 bp | This study |
| pHB163 | pHB158 containing the 475-bp NaeI-BamHI fragment from pHB161 with UAS C-1; contains UASs C-1 and C-2 + 10 bp | This study |
| pHB164 | pHB151 containing a 2.75-kb BamHI fragment from pHB109 with an hbpR-hbpC intergenic region-luxAB fusion; complete cbp-hbpR | This study |
| pHB165 | pHB150 containing a 2.75-kb BamHI fragment from pHB109 with an hbpR-hbpC intergenic region-luxAB fusion; incomplete cbp-hbpR | This study |
| pHB207 | Apr; pHB150 carrying the 2-kb BglII-SalI fragment from pHYBP132 which generated a complete hbpR without the CBP tag fusion | This study |
| pHB208 | pHB207 containing a 2.75-kb BamHI fragment from pHB109 with an hbpR-hbpC intergenic region-luxAB fusion; complete hbpR | This study |